I'm going to try and be extensive here since "josei" material is so hard to find.
+Hataraki Man
+Kuragehime
However the show you really want to watch is Otona Joshi no Anime Time, an anthology of four stories by female writers where the characters are between 28 and 40. The stories range from funny to bittersweet to disturbing. Once you've watched this, you'll wish more josei material made it to anime.
SHIROBAKO and the current Sakura Quest follow young women in their early to mid twenties. In the first, the ladies are trying to enter the anime industry; in Sakura Quest, they are trying to resuscitate tourism in a rural town.
Seirei no Moribito is a fantasy adventure set in what looks to be medieval Korea. The heroine, Balsa, is a 30-yo spear-wielding bodyguard who is tasked with protecting a young prince targeted for assassination. There's also an excellent live-action adaptation out there with material from the later novels; it's final season airs this fall.
Some of the characters in Death Parade are probably in their thirties, though the show isn't really about that stage of life like Otona Joshi is.
Genji Monogatari Sennenki is a beautifully-illustrated, though slow-moving adaptation of the famous "Tale of Genji" by the Lady Murasaki, considered the world's first novel. Genji himself is, of course, male, but the story concerns his various amorous relationships. Most of his romantic partners are in their twenties or older.
Kaguya-hime no Monogatari is an adaptation of the famous Japanese folk tale about a girl who was discovered inside a bamboo shoot. Much of the story takes place when she is in her twenties and of marriageable age.
Another recent film, Miss Hokusai, focuses on the famous painter Hokusai and his adult daughter who is probably in her twenties.
Kon Satoshi's film, Millennium Actress, follows the career of a B-movie actress over the course of twentieth century Japan. Most of the film takes place when she is in your target age range.
Maria in Junketsu no Maria is probably the right age, but she's a witch. She is seeking to use her powers to bring an end to the Hundred Years War. Minor ecchi and a romance.
Michiko to Hatchin is an adventure story about a woman in her mid-thirties who has escaped from prison. She grabs a young girl whom she believes is the daughter of her lost lover (and maybe her own child), and the two of them set off across Brazil in search of the guy.
Lupin III: Mine Fujiko to Iu Onna is another story about a female thief. Fujiko is a recurring character in the Lupin series, but here she gets a show of her own. Like Michiko, it's directed by Yamamoto Sayo, one of the few active and acclaimed female directors in anime. (She also made Yuri on ICE!!.)
Finally, I'll mention Yakushiji Ryoko no Kaiki Jikenbo, a stylish adventure starring the drop-dead gorgeous head of the "Supernatural Division" of the Tokyo Police and her male subordinate. The first half is a "monster-of-the-week" type of story, but there's more meat in second half.
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