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Ayame Nijou (二条菖蒲)Ayame, a widow who was married to a government official, is the person who gives her blood to Vivi since she owes him. She has romantic feelings for Vivi though revealed to be unrequited. Hana often gets jealous when she sees Vivi sucking Ayame's blood, wishing instead for him to suck hers. Chapter 27 shows how Vivi and Ayame met.
She convinced herself that she fell in love with him because of the way he smiled at Hana. She accepts the fact that she can't compete with Hana. In a recent chapter, Vivi told Ayame that he was through with sucking her blood.
She is always wearing a kimono.
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